a look into the unknown

To check this hypothesis, Kozak systematically mutated nucleotides around the initiation codon in a cloned rat preproinsulin gene. She substituted a synthetic ATG-containing oligonucleotide for the normal initiating ATG, then introduced mutations into this initiation region, placed the mutated genes under control of the SV40 virus promoter, introduced them into monkey(COS) cells, then labeled newly synthesized proteins with [35S] methionine, immunoprecipitated the proinsulin, electrophoresed it, and detected it by fluorography, a technique akin to autoradiography. Finally, she scanned the fluorograph with a densitometer to quantify the production of proinsulin. The better translation initiaition, the more proinsulin was made.

Um.. i heard North Campus is a nice place..